【Objective】 This study aims to examine the expression pattern of the PYL gene in Luffa cylindrica after ABA treatment. 【Methods】 Bioinformatics methods were used to analyze the distribution, chromosome location, gene structure, gene collinearity, selection pressure analysis, evolutionary relationships, and cis-acting elements of the PYL gene family in the whole genome of Luffa cylindrica cucumis. The expression specificity of the PYL gene family under different concentrations of ABA was then verified by qRT-PCR. 【Results】 Eleven LcPYL genes were identified within the entire genome of Luffa cylindrica. These genes correspond to CDS lengths of 576-966 bp and all possess typical PYR_PYL_RCAR-like structures. Collinearity analysis reveals that 7 pairs derived from genome-wide duplication events are expected to have similar biological functions. The PYL gene promoter in Luffa cylindrica contains several cis-acting elements associated with plant hormones, and the expression of its members shows certain tissue specificity. The expression of LcPYLs in Luffa cylindrica fruit did not exhibit significant changes when treated with 2 mg/ml ABA, However, the expression of LcPYL5, LcPYL7 and LcPYL11 significantly increased after 4 mg/ml ABA treatment, LcPYL1 and LcPYL7 were significantly expressed on the day of flowering post 8 mg/ml ABA treatment. 【Conclusion】 In this investigation, we conducted a systematic identification and analysis of PYL family members within Luffa cylindrica in order to establish their molecular structure and expression patterns. Our findings suggest that LcPYL1, LcPYL5, LcPYL7, and LcPYL11 are potentially crucial genes that are closely linked to endogenous ABA biosynthesis.