【Objective】 The study aims to explore the physiological mechanism of different grafting patterns to alleviate the low temperature stress of melon(Cucumis melo L.) seedlings, in order to provide a theoretical basis for selecting appropriate grafting patterns to improve the low temperature resistance of melon in production practice. 【Methods】 Taking thick skin melon varieties ''Early Honey 1'', ''Snow Pear'' and thin skin melon variety ''Sesame Honey'' as scions, and white seed pumpkin as rootstock, and taking melon self rooted seedling as controls, the effects of double root grafting, adhering and attaching on the photosynthetic pigment content, photosynthetic gas exchange parameters, cell membrane permeability and antioxidant enzyme activity of various melon seedlings leaves before low temperature(4 ℃), low temperature and after low temperature treatment (rewarming treatment) were studied. 【Results】 (1) The photosynthetic pigment content (chlorophyll and carotenoid) and photosynthetic gas exchange parameters (net photosynthetic rate, stomatal conductance, transpiration rate, intercellular CO2 concentration) of various melon varieties and grafted seedlings leaves were significantly decreased after low temperature treatment, among which the grafted seedling decreased rate of attaching treatment was the lowest, and all treatments increased after rewarming, and the increase range of attaching treatment was the highest. (2) The relative conductivity (REC), malondialdehyde (MDA) content, hydrogen peroxide (H2O2) content and superoxide anion (O2·-) production rate in leaves of all kinds of grafted seedlings of all melon varieties under low temperature treatment were significantly increased compared with those before low temperature treatment, and the attaching grafted seedlings had the lowest increase. The REC, MDA content, H2O2 content and O2·- production rate in leaves of all kinds of grafted seedlings of all melon varieties under rewarming treatment were decreased compared with those low temperature, and the attaching grafted seedlings had the highest decrease. (3) The activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) in leaves of all kinds of grafted seedlings of all melon varieties under low temperature were significantly increased compared with those before low temperature treatment, and these antioxidant enzyme activities in leaves of all kinds of grafted seedlings of all melon varieties under rewarming treatment were significantly increased compared with those low temperature treatment, and the attaching grafted seedlings had the most significant increase. 【Conclusion】 The three grafting patterns can significantly enhance the activities of antioxidant enzymes of thick-skinned and thin-skinned melon seedlings under low temperature stress and rewarming, effectively reduce the accumulation of reactive oxygen species, maintain the photosynthetic capacity of seedlings, and improve the low temperature tolerance of seedlings, and the attaching grafting pattern has the best performance.