【Objective】 Sugars will eventually be exported transporters (SWEETs) play an important role in the growth and development of plants. The aims of the study were to elucidate the role of SWEETs genes family in sugar accumulation during the development of wolfberry, and provide a reference for revealing the role of SWEET gene in the development of Lycium barbarum L. fruit. 【Methods】 In the present study, based on genomic data of L. barbarum L., the SWEET gene (LbaSWEET) of L. barbarum L.was identified by bioinformatics method, and the expression of LbaSWEET during fruit development was analyzed using published transcriptional data. 【Results】 The results showed that there were 37 members of the SWEET gene family, which were randomly distributed on 10 chromosomes, encoding 152-621 amino acids, respectively. The molecular weight of the protein was 16.87-69.97 kD, and the isoelectric point was 4.96~9.86. Subcellular localization is predicted to be located in the chloroplast or plasma membrane, most containing seven transmembrane helices. Phylogenetic analysis indicated that 37 LbaSWEET proteins could be divided into four subgroups, each of which had similar gene structure and conserved motif composition. The analysis of promoter elements showed that LbaSWEET gene promoter was rich in hormone response, stress response and growth response elements. Transcriptome data and qRT-PCR analysis showed that the expressions of LbaSWEET9 and LbaSWEET29 genes increased significantly with fruit ripening. Pearson correlation analysis showed that the expression levels of LbaSWEET9 and LbaSWEET29 genes were significantly positively correlated with fructose content. 【Conclusion】 It is speculated that LbaSWEET9 and LbaSWEET29 are the key genes that played an important role in the accumulation of fructose sugar.