茶树NAC转录因子CsNAC79和CsNAC9的鉴定及对非生物胁迫的响应分析
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1.南京农业大学园艺学院;2.南京农业大学

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Q785Q786Q789???????

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国家自然科学基金(31870681);江苏省政策引导类计划(SZ-LYG202126);江苏高校优势学科建设项目(PAPD)


Identification and response to different abiotic stress of NAC transcription factors CsNAC79 and CsNAC9 in tea plants
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    摘要:

    【目的】文章旨在鉴定茶树NAC转录因子基因CsNAC79和CsNAC9并分析它们在干旱、盐、高温、低温、外施ABA和外施GA3胁迫下的表达模式,为进一步解析CsNAC79和CsNAC9转录因子的生物学功能提供参考。【方法】以茶树‘龙井43’为材料,采用RT-PCR扩增NAC转录因子家族基因CsNAC79和CsNAC9,并进行生物信息学分析;采用实时荧光定量PCR检测CsNAC79和CsNAC9在茶树6种非生物胁迫下的相对表达量。【结果】CsNAC79和CsNAC9编码409个和282个氨基酸,且分别在15—150氨基酸位点、11—134氨基酸位点含有NAC家族成员典型的NAM保守结构域;CsNAC79蛋白与中华猕猴桃、柿、洋蓟亲缘关系较近,CsNAC9蛋白与桃、荔枝、榴莲亲缘关系较近;2个NAC转录因子均是亲水性蛋白,皆不含信号肽和跨膜结构;CsNAC79和CsNAC9的启动子区域都含有非生物胁迫响应元件;蛋白质空间结构分析显示,CsNAC79和CsNAC9蛋白主要是由不规则卷曲和α螺旋组成;表达分析表明:CsNAC79和CsNAC9基因在6种不同非生物胁迫下均上调表达。【结论】茶树CsNAC79和CsNAC9基因响应多种非生物胁迫。

    Abstract:

    【Objective】 NAC transcription factor is a plant-specific transcription factor family, which is involved in plant biotic and abiotic stress response and hormone signal transduction. 【Methods】 In this study, two NAC transcription factor genes CsNAC79 and CsNAC9 were cloned from tea cultivar ''Longjing 43''. The analysis showed that CsNAC79 and CsNAC9 transcription factors contained typical NAM conserved domains of NAC family members at 15-150 amino acid sites and 11-134 amino acid sites, respectively. Phylogenetic tree analysis showed that CsNAC79 was closely related to Actinidia chinensis, Diospyros kaki and Cynara scolymus; CsNAC9 was closely related to Amygdalus persica, Litchi chinensis and Durio zibethinus. Amino acid hydrophilicity / hydrophobicity analysis showed that both NAC transcription factors were hydrophilic proteins without signal peptides and transmembrane structures. Prediction analysis of cis-acting elements in the promoter region showed that both NAC transcription factors contained abiotic stress response elements. Protein spatial structure analysis showed that CsNAC79 and CsNAC9 proteins were mainly composed of random coils and alpha helices. The expression levels of CsNAC79 and CsNAC9 genes under different abiotic stresses were detected and analyzed by RT-qPCR. The results showed that the expression levels of CsNAC79 and CsNAC9 genes was up-regulated by drought, high salt, high temperature, low temperature, exogenous ABA and exogenous GA3 treatments, indicating that CsNAC79 and CsNAC9 genes were related to abiotic stress resistance response. In summary, CsNAC79 and CsNAC9 genes in tea plants may respond to various abiotic stresses. This study provides a reference for further analysis of the biological functions of CsNAC79 and CsNAC9 transcription factors in the future.

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孔洁玙,杨妮,罗微,等.茶树NAC转录因子CsNAC79和CsNAC9的鉴定及对非生物胁迫的响应分析[J].西北植物学报,2024,44(4):572-581

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  • 收稿日期:2023-09-20
  • 最后修改日期:2023-12-13
  • 录用日期:2023-12-25
  • 在线发布日期: 2024-03-28
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