【Objective】 Frost temperature thresholds of grape are the basis for frost monitoring, prediction, assessment and prevention. The study was conducted to investigate the low-temperature tolerance of the main wine grape ‘Cabernet Sauvignon’ at different development stages in the eastern foothills of Helan Mountain, and further to establish a group of frost temperature thresholds for wine grapes. The study aimed to provide the theory basis for frost prediction and prevention of wine grapes in the eastern foothills of Helan Mountain. 【Methods】 We used a field frost chamber to treat the pompom, bud, new leave, and new shoot of the main wine grape variety ''Cabernet Sauvignon'' at different developmental stages with low temperatures in the eastern foothills of the Helan Mountainduring the period of April to May 2022. The supercooling point, relative electrical conductivity (REC) and semi-lethal temperature (LT50) were measured for each treatment. And we carried out a follow-up field survey on frozen ratio after various low-temperature treatment. Then, the regression model based on the correlation between frozen ratio and low temperature was set up to calculate the critical temperatures for light, moderate and severe frost at different development stages of wine grape. The abovementioned indicators at the critical temperature of frost were comprehensively analyzed to determine the frost temperature thresholds at different development stages of ''Cabernet Sauvignon''. 【Results】 The results showed that: The low-temperatures had different effects on ''Cabernet Sauvignon'' during the pompom, bud opening, leaf spreading and new shoot growth stages. The lower the temperature was, the higher the frozen ratio at the different stages was. The supercooling point and the semi-lethal temperature (LT50) were gradually increasing as the development of wine grape progresses. The low temperature tolerance in the pompom stage was the strongest and followed in the bud opening and leaf spreading stages. The tolerance of new shoot growth stages was the weakest. 【Conclusion】 Research suggested that -7.70℃ is the turning point for severe frost at the pompom stage, while the critical temperature of bud opening stage was -4.49℃. The temperature lower than -2.79℃ at the leaf spreading stage bright irreversible damage to the wine grape. Furthermore, a low temperature below -1.62℃ in the new shoot growth stage caused severe frost.