外源IBA对无花果扦插苗生理特性及内源IAA生物合成途径的影响
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1.云南农业大学 园林园艺学院;2.新疆农业科学院 吐鲁番农业科学研究所

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云南省基础研究专项-青年项目(202101AU070094),云南省基础研究专项-面上项目(202301AT07049)。


Effects of exogenous IBA on physiological characteristics and endogenous IAA biosynthesis pathway of Fig cuttings
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    摘要:

    【目的】随着区域栽培环境的变化,扦插苗生根难问题逐渐凸显。探讨适宜浓度吲哚丁酸(IBA)对无花果插穗生根萌芽、抗氧化性及生长素生物合成途径相关基因表达的影响,可为其应用于无花果的育种、繁殖、推广和种植提供理论依据。【方法】以‘波姬红’无花果品种硬枝为插穗,分析不同质量浓度IBA(0,30,45,60,90 mg/L)处理对插穗生根性状、抗氧化特性的影响,并对45 mg/L IBA处理及对照组的扦插枝条中段的腋芽进行转录组分析。【结果】(1)无花果插穗萌芽率和生根率在45 mg/L IBA处理时达到最大值,并与其他处理和对照差异显著。(2)随着IBA浓度的增加,插穗SOD和CAT活性先下降后上升,并均在45,60 mg/LIBA处理下显著低于对照,而POD活性无显著变化;各浓度IBA处理插穗中MDA和H2O2含量均显著高于对照,且45 mg/L IBA处理MDA显著低于其余处理。(3)45 mg/L IBA处理及对照组中共存在6 879个差异表达基因,KEGG富集显示有10个差异途径,GO富集分析表明生物学过程和分子功能为主要的生物学途径;与CAT、SOD相关的基因集中富集在过氧化物酶体通路上,POD相关基因则富集在苯丙烷生物合成通路中;IAA生物合成途径中代谢相关基因FcGH3显著上调表达,与信号转导相关基因FcAUX1、FcARG7和FcARF等显著下调表达。【结论】外源IBA处理会导致无花果插穗抗氧化酶和IAA生物合成途径中相关基因表达的差异变化,增强插穗抗逆性,促进插穗生根、萌芽、成苗,并以外源45 mg/L IBA促进效果最好。

    Abstract:

    【Objective】 With the change of regional cultivation environment, the problem of rooting difficulty of cuttings is gradually highlighted. Exploring the effects of suitable concentration of indole butyric acid (IBA) on rooting and sprouting, antioxidant property and gene expression related to growth hormone biosynthesis pathway of fig(Ficus carica) cuttings may provide theoretical basis for its application in fig breeding, propagation, promotion and planting. 【Methods】 The hard branches of ''Brown Turkey'' fig variety were used as cuttings to analyze the effects of different mass concentrations of IBA (0, 30, 45, 60, 90 mg/L) treatments on the rooting traits and antioxidant properties of the scions, as well as transcriptome analyses of axillary buds in the middle part of fig cuttings from 45 mg/L IBA treatments and the control group. 【Results】(1) The germination and rooting rates of fig spikes reached the maximum at 45 mg/L IBA treatment and differed significantly from other treatments and control.(2) With the increase of IBA concentration, the SOD and CAT activities of spike showed the trend of decreasing and then increasing, and both were significantly lower than the control in 45 and 60 mg/L IBA treatment, while POD activity was not significantly different between the treatment and the control; the content of MDA and H2O2 in the spikes of each concentration of IBA treatment was significantly higher than that of the control, and the MDA content in the spikes of 45 mg/L IBA treatment was significantly lower than the rest of the treatments. (3) The results of transcriptome analysis showed that there were 6 879 differentially expressed genes in 45 mg/L IBA treatment and control, and KEGG enrichment showed that there were 10 differential pathways, and GO enrichment analysis indicated that the biological processes and molecular functions were the main biological pathways; the genes related to CAT and SOD were enriched in peroxisomal pathway, while the genes related to POD were enriched in phenylpropane biosynthetic pathway. The metabolism-related gene FcGH3 was significantly up-regulated in the IAA biosynthesis pathway, and the genes related to signaling, such as FcAUX1, FcARG7 and FcARF, were significantly down-regulated. 【Conclusion】 Exogenous IBA treatment led to differential changes in the expression of relevant genes in the antioxidant enzymes and IAA biosynthesis pathway of the fig spikes, enhanced the spikes'' resistance to stress, and promoted the spikes'' rooting, sprouting and seedling formation. Exogenous 45 mg/L IBA treatment had the best effect.

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陈紫玉,巴哈依丁·吾甫尔,任桂霖,等.外源IBA对无花果扦插苗生理特性及内源IAA生物合成途径的影响[J].西北植物学报,2024,44(7):1046-1054

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  • 收稿日期:2023-10-24
  • 最后修改日期:2024-03-08
  • 录用日期:2024-03-11
  • 在线发布日期: 2024-05-31
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