茶树种质叶片色素含量与叶色参数的关系
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丽水市农林科学研究院

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中国农业科学院茶叶研究所长三角创新中心项目建设(SYCZHT-01);浙江省农业(茶树)新品种选育重大科技专项(2021C02067-6);2022年市级农科院联盟区域示范性项目-“浸入式水肥自供一体育苗机组及其育苗关键技术试验”(2022SJLM20)


Relationship between leaf pigment content and leaf color parameters in tea tree germplasm
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    摘要:

    为了探究茶树种质资源叶片色素含量与叶色参数之间的关系,以143份黄、白化和紫化叶色特异茶树种质为材料,比较分析各种质叶片叶色参数L*、ɑ*、b*值和花青素、叶绿素、类胡萝卜素含量差异,并通过主成分分析、聚类分析、相关性分析、多元逐步回归分析和通径分析探讨叶片色素含量和叶色参数关系,为叶色特异茶树种质叶片呈色机理及品种选育提供依据。结果表明:(1)紫化茶树种质花青素含量和ɑ*值较高,叶片呈现紫红色;黄化种质类胡萝卜素含量/叶绿素含量比值和b*值较高,叶片呈黄色,白化种质L*值较高,叶片呈白色。在叶色表型性状方面,叶色特异茶树种质大体聚为两大类群,白色系和黄色系聚为一大类,紫色系聚为另一大类。(2)叶色特异茶树种质叶片叶色参数ɑ*值与花青素含量呈极显著正相关,L*值、b*值与花青素和叶绿素含量均呈极显著负相关,且b*值与类胡萝卜素含量呈极显著正相关;花青素、叶绿素对叶片CIEL*ɑ*b*值直接作用较大。总之,ɑ*值可作为描述叶色特异茶树种质叶片紫红色性状的代表性参数,b*值可作为描述叶色特异茶树种质叶片黄色性状的代表性参数。

    Abstract:

    In order to explore the relationship between leaf pigment content and leaf color parameters of tea germplasm resources, 143 tea germplasms with yellow,albino and purple variation in leaf color were selected, and the differences of the values of L*,a* and b* of leaf color parameters and the pigment contents of total anthocyanin, chlorophyll, carotenoid in various qualitative leaves were compared and analyzed.The relationship between leaf pigment content and leaf color parameters were explored through principal component analysis,cluster analysis,correlation analysis, multiple stepwise regression analysis, and path analysis to provide the basis for leaf color mechanism of tea germplasms and variety breeding.The results showed that:(1) The content of anthocyanin and a* value in purple tea germplasms were higher, and thecolor of leaves showed purple red.The ratio of carotenoid content to chlorophyll content and b* value in yellow germplasms were higher, and the color of leaves were yellow. The L*value of albino germplasms was higher and the color of leaves were white.In terms of leaf color phenotypic traits, leaf color specific tea germplasms generally clustered into two groups, white and yellow in one group and purple in the other group. (2) The leaf color parameter a* value of leaf color specific tea tree germplasms in tea leaves exhibited a significantly positive correlation with anthocyanin content, while L* value as well as b* value had extremely significant negative correlation with anthocyanin and chlorophyll content, and there were a very significantly strong negative correlation between b* value and carotenoid content.Anthocyanin and chlorophyll contributed more directly to the leaf CIEL*ɑ*b* value.In conclusion,ɑ* value can be used as a representative parameter to describe the leaf purple-red traits of leaf color specific tea tree germplasms and b* value to describe the leaf yellow traits of leaf color specific tea tree germplasms.

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宁秋燕,冯亚颀,何卫中,等.茶树种质叶片色素含量与叶色参数的关系[J].西北植物学报,2024,44(6):870-879

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  • 收稿日期:2023-11-03
  • 最后修改日期:2024-02-05
  • 录用日期:2024-03-04
  • 在线发布日期: 2024-04-24
  • 出版日期: 2024-07-01
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