几丁质和flg22诱导的辣椒幼苗先天免疫生理响应特征
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四川省农业科学院园艺研究所/蔬菜种质与品种创新四川省重点实验室/农业农村部西南地区园艺作物生物与种质创制重点实验室

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国家现代农业产业技术体系四川省创新团队(sccxtd-2020-05);四川省科技计划青年创新团队(2021JDT0006); “十四五”四川省蔬菜育种公关项目(2021YFYZ0022); 四川省自然科学基金青年基金项(2023NSFSC1242);四川省财政自主创新专项项目(2022ZZCX45)。


Induction of innate immune physiological responses in pepper seedlings by chitin and flg22
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    摘要:

    【目的】明确几丁质和鞭毛蛋白衍生肽flg22诱导的辣椒幼苗先天免疫生理响应特征,探讨辣椒先天免疫生理响应与辣椒抗多种病害的关系。【方法】以5个四川本地辣椒品种幼苗为试材,鉴定它们的青枯病和疫病病情指数和抗性水平,采用水培法培育幼苗并进行外施几丁质和flg22处理,检测各品种不同诱导时间下幼苗根系生长、气孔孔径、胼胝质沉积、活性氧(ROS)积累和SOD、CAT活性,以及先天免疫相关基因表达量的变化,并运用生物统计学综合评价其生理响应及其与抗病性关系。【结果】(1)幼苗青枯病和疫病病情指数以‘川腾10号’最低,抗病性最强,以‘本地条椒’最高,抗病性最弱。(2)外源几丁质和flg22抑制了各品种幼苗根系生长速率,诱导离体叶片气孔闭合,促进叶片细胞壁胼胝质沉积加厚,ROS含量持续增加,SOD和CAT活性不断提高。各品种幼苗先天免疫生理响应指标的平均隶属函数值以‘川腾10号’最高,‘本地条椒’最低,并且平均隶属函数值与疫病、青枯病病情指数均具有显著负相关性。(3)外源flg22和几丁质诱导‘川腾10号’幼苗先天免疫相关基因CaWRKY22、CaMAPK7和ChiIV3显著上调表达。【结论】外源flg22和几丁质可诱导辣椒幼苗先天免疫生理响应,且响应程度强弱在品种间存在差异,依据生理响应指标通过隶属函数可综合评价辣椒品种的抗病水平;‘川腾10号’的平均隶属函数值最高,多抗性水平相对最好,这与其先天免疫相关基因CaWRKY22、CaMAPK7和ChiIV3的显著上调表达有关。

    Abstract:

    [Objective] To clarify the characteristics of innate immune physiological responses induced by chitin and flagellin-derived peptide flg22 in pepper seedlings, and to explore the relationship between innate immune physiological responses and pepper multiple resistance to diseases. [Methods] Five local pepper cultivars from Sichuan were used as experimental materials in this study. The disease indexes of bacterial wilt and phytophthora blight in pepper seedlings were analyzed. Meanwhile seedlings were cultured by hydroponics and treated with chitin and flg22 exogenously. Among different pepper cultivars, under different induction times, seedling root growth, stomata aperture, callose deposition, reactive oxygen species (ROS) accumulation, SOD, CAT activities as well as the changes of related gene expression were checked . Then the physiological responses and theirs relationship with disease resistance were evaluated comprehensively by using biostatistics.[Results] (1) The results showed that the lowest disease index of bacterial wilt and phytophthora blight was 'CT10' cultivar, was the strongest disease resistance, and the highest disease index of bacterial wilt and phytophthora blight was 'TJ' cultivar, was the weakest disease resistance.(2) Exogenous chitin and flg22 inhibited the root growth rate of pepper seedlings in five cultivars, induced stomatal closure on detached leaves, promoted the accumulations of callose deposition in leaf cell wall, increased ROS content constantly with improved SOD and CAT activities continuously. By calculating the average membership function value of the innate immune physiological response index of each different cultivar, it showed that the highest was 'CT10' and the lowest was 'TJ'. The average membership function value has a significant negative correlation with the disease index of bacterial wilt and phytophthora blight .(3) Exogenous flg22 and chitin induced some innate immune involved genes of 'CT10' seedlings significantly upregulation expressions, like CaWRKY22, CaMAPK7 and ChiIV3.[Conclusion] Exogenous flg22 and chitin induced the innate immune physiological responses in pepper seedlings, and the strength of the responses showed different among different varieties. Based on the membership function, the disease resistance level of pepper varieties was evaluated comprehensively. The results showed the average membership function of 'CT10' was the highest and the multi-resistance level was the best, which was related to the significant up-regulated expression of innate immune involved genes CaWRKY22, CaMAPK7 and ChiIV3.

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巩雪峰,许艺,宋占锋,等.几丁质和flg22诱导的辣椒幼苗先天免疫生理响应特征[J].西北植物学报,2024,44(8):1190-1200

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  • 收稿日期:2023-11-07
  • 最后修改日期:2024-04-26
  • 录用日期:2024-04-30
  • 在线发布日期: 2024-06-24
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