[Objective] To identify and analyze the members of the tonoplast intrinsic proteins (TIP) subfamily in apple, and to investigate the expression patterns under drought stress. [Methods] The whole genome of apple MdTIPs was identified by bioinformatics methods, and the physicochemical properties, gene structures, conserved motifs, cis-regulatory elements, phylogenetic trees, etc. of the subfamily members were analyzed, and the expression patterns of the MdTIPs were also analyzed under drought stress in different organs by real-time fluorescence quantitative PCR (qRT-PCR). [Results] A total of 13 MdTIP genes were identified in the apple genome, most of members were subcellularly localized on the plasma membrane. The chromosome localization suggested that all members were distributed on 10 chromosomes, with 1-3 members localized on each chromosome. Besides, the promoter regions of the subfamily members contained a variety of response elements in response to hormonal and adversity stresses. The qRT-PCR showed that MdTIPs subfamily members were up-regulated in roots except MdTIP1;1, of which MdTIP1;3 and MdTIP1;4 were up-regulated 5.27 times and 5.69 times, respectively, compared with the control. Therefore, these two genes were presumed to be the key genes involved in the regulation of drought stress. [Conclusion] Preliminary identification and information of MdTIPs subfamily members were provided. 10 MdTIPs subfamily members were differentially expressed in roots, stems and leaves, and 12 members were highly expressed in roots. The results provide valuable information for further research on utilization of drought resistance gene resources in apple.