In order to reveal the molecular mechanism of photosynthetic midday depression breaking by water spraying, the effects of continuous spraying on mango leaves were studied by omics technology. This study can provide scientific basis for further optimizing water management to achieve the best results, , and will have theoretical significance for improving mango yield and quality by responding to the national policy of reducing fertilizer and increasing efficiency. 【Methods】 The mango cultivar "Tainong No. 1" in Yuanjiang was used as the experimental material. The mango in the fruiting stage was sprayed by water for three times during the photosynthesis midday depression (12:30-14:10), 20 minutes each time, with no water spraying as the control. Then the leaves collected from treatment and control were conducted for transcriptome sequencing analysis by The Illumina Hi SeqTM 2000. The gene ontology(GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases were used to analyze the differentially expressed genes (DEGs) between the two groups. Differential analysis of gene expression was conducted by using DESeq, with |log2αFC| >1 and P<0.05 as the conditions for screening the DEGs. 【Results】 3 789, 2 885, and 1 667 differentially expressed genes were obtained separately from three development stages. GO analysis found that the DEGswere highly correlated with the membrane, intrinsic components of the membrane, components of the membrane and other items in the classification of cell components. KEGG analysis showed that the DEGs were mainly enriched in the pathways of plant hormone signal transduction, plant MAPK signal pathway, plant circadian rhythm, metabolism of amino sugar and nucleotide sugar, flavonoid biosynthesis, photosynthetic biological carbon fixation, biosynthesis of cutin, sulfite and wax, metabolism of porphyrin and chlorophyll, starch and sucrose metabolism, and glyceride metabolism. According to the enrichment results, eight differentially expressed genes were screened. Through quantitative real-time PCR (qRT-PCR) verification, the gene expression trend was roughly consistent with the results of RNA-seq. 【Conclusion】The delayed expression of CAO and POR genes suggests that water spraying can prolong the synthesis time of chlorophyll; the gene expression of FBPase and SBPase slightly decreased, suggesting that the spraying treatment might regulate the expression of genes and relieve the midday depression of photosynthetic, which may be beneficial to the carbon assimilation and photosynthetic efficiency.