【Objective】 The purpose of present study is to clarify the quantitative relationship between the hydraulic conductance of vine stem segments and temperature, and to explore the influence of nods on water transport efficiency and safety. 【Methods】 The water conductivity (K), sapwood specific conductivity (Ks) and percent loss of conductivity (PLC) of internode and single-node stem segments of Vitis vinifera L.× V. labrusca L. were measured by low pressure flow meter method with the temperature range of 0.5-40 ℃, and the differences of water conductivity between the two segments were analyzed. 【Results】 The K and Ks of Vitis vinifera L.× V. labrusca L. stem segment had significant linear relationship with temperature. As temperature raised from 0.5 ℃ to 40℃, the initial conductivity, maximum conductivity, initial Ks and maximum Ks of internode stem increased 154%, 198%, 157% and 200%, respectively. The K and Ks values in internode stem segment were higher than those in single-node stem segment, and the difference increased with the increase of temperature. The PLC of internode stem segment was significantly greater than that of single-node stem segment, while the water conductivity and specific conductivity of single-node stem segment were less affected by temperature than that of internode stem segment. The linear regression equation can be used to predict the K and Ks values at different temperatures. As the K and Ks being tradeoff against the viscosity coefficient of the water at each temperature, it showed no significant difference among the tradeoff water conductivity (K′) and sapwood specific conductivity (Ks′) at different temperatures. 【Conclusion】 The K and Ks of V. vinifera L.× V. labrusca L. stem increased with the rise of temperature, and the node can tradeoff the efficiency and safety of the stem water transport.