【Objective】To investigate the pollen biology characteristics of Primulina guizhongensis and the influence of the breeding system on its sexual reproduction, uncovering its reproduction mechanism and providing theoretical references for the conservation and development of Primulina.【Methods】Using P. guizhongensis as the material, we observed the floral characteristics of P. guizhongensis, screened the pollen isolation medium and the best pollen pollen viability detection method, explored optimal pollen storage conditions, determine the type of its breeding system.【Results】(1) P. guizhongensis dichogamy, stamens ripening before pistils; herkogamy, pistils higher than stamens (2) The optimum medium formulation for the culture of isolated pollen germination is 40 mg/mL boric acid + 10% sucrose + 7 g/L agar for two hours. (3) Pollen maintained a high germination rate after 7 d of storage at both -20°C and -80°C (4) The stigmas of P. guizhongensis are most fertile at 2-4 d of flowering. (5) P/O is 518.36~782.75, OCI is 5. (6) P. guizhongensis has a high self-fertilisation affinity and is unable to undergo fusionless reproduction.【Conclusion】 The in vitro germination method is the most intuitive and accurate way to detect pollen viability, low temperatures are conducive to pollen storage of P. guizhongensis, and the breeding system is predominantly heterogametic.