甘蓝型油菜种子硫代葡萄糖苷含量的QTL定位及候选基因鉴定
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1.安康学院;2.陕西省杂交油菜研究中心

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Q347.1

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陕西高校青年创新团队项目(2023AYTD01),陕西省大学生创新创业训练计划项目(S202311397010),陕西省重点研发计划项目(2020ZDLNY04-01),安康学院高层次人才引进项目(2021AYQDZR12)。


QTL mapping and Candidate Gene Identify of Seed Glucosinolate Content in Brassica napus
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    摘要:

    【目的】为了解析油菜种子硫代葡萄糖苷性状的重要遗传位点及候选基因,【方法】本研究利用KN DH群体在冬性环境2015-2018连续4年的种子硫苷含量表型和KN 高密度SNP遗传连锁图谱,通过Wincart 2.5软件的符合区间作图法对甘蓝型油菜种子硫代葡萄糖苷含量进行QTL定位和潜在候选基因鉴定。【结果】共鉴定到47个硫苷含量QTL,单个QTL解释表型变异最大是qGC.16YL19-4(19.44%),解释表型变异最小的是qGC.15YL12-5(1.82%)。利用元分析的方法将初步鉴定的47个QTL整合为38个consensus QTL,其中7个consensus QTL(cqGC.A9-5、cqGC.A9-7、cqGC.A9-9、cqGC.C2-9、cqGC.C2-10、cqGC.C9-5和cqGC.C9-6)为环境稳定表达QTL,包括3个硫苷含量主效QTL(cqGC.A9-5、cqGC.C2-10和cqGC.C9-5)。在主效QTLcqGC.A9-5和cqGC.C9-5鉴定到3个候选基因BnaA09g05480D,BnaC09g05620D和BnaC09g05810D,其功能主要涉及了油菜硫苷生物合成途径中吲哚-3-乙醛肟(IAOx)的合成和将2-烷基-苹果酸异构化形成3-烷基-苹果酸酯,以及硫苷的转运与分配。【结论】本研究获得了油菜种子硫苷含量3个主效QTL及3个候选基因,该结果为硫苷含量相关基因的功能机械和优质油菜品种培育提供理论依据。

    Abstract:

    【Objective】To explore the genetic loci and candidate genes of glucosinolate content in Brassica napus, 【Methods】 The phenotype data for glucosinolate content of a double haploid (DH) population named KN population was surveyed in the year 2013-2017 for QTL mapping and identification of candidate genes using composite interval mapping by Wincart 2.5 software based on the high-density SNP genetic linkage map. 【Results】A total of 47 identified QTLs for glucosinolate content were detected, individual QTLs could explain 2.48%–19.63% of the phenotypic variation, of which qGC.16YL19-4 was the maximum phenotypic variation of 19.44% and qGC.15YL12-5 was the minimum phenotypic variation of 1.82%. 47 identified QTLs were integrated into 38 consensus QTL by meta-analysis. Seven environment stable expression QTLs (cqGC.A9-5, cqGC.A9-7, cqGC.A9-9, cqGC.C2-9, cqGC.C2-10, cqGC.C9-5 and cqGC.C9-6) for glucosinolate content were identified, including three major QTLs (cqGC.A9-5, cqGC.C2-10 and cqGC.C9-5). Three candidate genes (BnaA09g05480D, BnaC09g05620D and BnaC09g05810D) within major QTL confidence interval were identified, which were located in two major QTL regions. Based on their annotation, these candidate genes involved in the biosynthetic pathway of glucosinolates (the biosynthesis of IAOx and isomerization of 2-alkyl malic acid to form 3-alkyl malic acid ester) and transport and distribution of glucosinolates. 【Conclusion】 Three major QTLs for glucosinolate content and three candidate genes were identified, these results lay the provide theoretical basis for functional analysis of genes and the cultivation of fine quality rapeseed varieties in the future.

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  • 收稿日期:2024-02-24
  • 最后修改日期:2024-04-17
  • 录用日期:2024-04-18
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