谷子CPP基因家族鉴定及外源硒响应分析
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河南科技大学农学院/河南省旱地作物种质资源利用工程研究中心

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Q789;S515

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河南省产业科技特派员项目(2018HNCYTPY01);河南省旱地绿色智慧农业特色骨干学科群建设项目(17100001)


Identification of CPP Gene Family and Analysis of ExogenousSelenium Response in Foxtail Millet
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    摘要:

    【目的】探究谷子CPP家族成员基本特征及其在外源硒处理下的响应模式,为谷子富硒高叶酸新品种选育提供新的遗传材料。【方法】借助生物信息学工具鉴定谷子CPP家族成员,采用qRT-PCR技术对CPP基因在不同组织及外源硒处理下的表达水平进行检测,为揭示谷子CPP家族生物学功能及外源硒响应机制奠定基础。【结果】(1)谷子基因组中包含9个CPP基因,定位于6条染色体上,依照基因在染色体的排布命名为SiCPP1~SiCPP9,氨基酸数目在220~899之间,相对分子量为23624.32~97253.16Da,等电点介于6.64~9.17之间,内含子数目为3~11个不等,亚细胞预测显示该家族所有成员均能在细胞核定位到,蛋白质二级结构预测表明该家族中占比最大的是α-螺旋其次是无规则卷曲。(2)系统进化分析表明,谷子CPP蛋白可分为4个亚家族,相同亚家族之间保守基序和结构域的数目及分布相似。(3)启动子分析发现谷子CPP家族中存在大量光、生长发育、激素以及胁迫响应元件。(4)荧光定量结果显示,谷子CPP家族成员在根、茎、叶和穗中差异表达。谷子CPP家族9个成员均对外源硒存在响应,其中SiCPP5、SiCPP6、SiCPP7和SiCPP8响应程度最强。【结论】谷子CPP家族成员具有组织表达特异性且对外源硒存在不同程度响应。

    Abstract:

    【Objective】To explore the basic characteristics of CPP family members of foxtail millet and their response mode under exogenous selenium treatment, and to provide new genetic materials for the breeding of innovative varieties rich in selenium and high folate in foxtail millet. 【Methods】Using bioinformation tools to identify CPP family members, using qRT-PCR technology to determine CPP gene expression in various organizations and in exogenous selenium, which laid a foundation for revealing the biological function of CPP family and the response mechanism of exogenous selenium in foxtail millet. 【Results】(1) The millet genome contained 9 CPP genes, which were located on 6 chromosomes and were named SiCPP1-SiCPP9 according to the arrangement of genes in chromosomes. Amino acids ranged from 220 to 899, the relative molecular weight ranged from 23624.32 to 97253.16Da, and the isoelectric point ranged from 6.64 to 9.17, the number of introns ranged from 3 to 11, subcellular predictions showed that all family members could position themselves in nucleus. The prediction of the secondary structure of the protein indicated that the proportion of irregular curling and α-helix in the family was the heaviest.. (2) Phylogenetic analysis showed that gluten proteins could be divided into four subfamilies, the number and distribution of conservative elements and structural fields among the same subclans. (3) Promoter analysis revealed a large number of light, growth and development, hormone and stress response elements in foxtail millet CPP family. (4) Fluorescence quantitative results showed that CPP family members were differentially expressed in root, stem, leaf and ear.All 9 members of the millet CPP family were responsive to exogenous selenium, among which SiCPP5, SiCPP6, SiCPP7 and SiCPP8 were the most responsive. 【Conclusion】Members of the millet CPP family had tissue expression specificity and varying degrees of response to exogenous selenium.

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  • 收稿日期:2024-03-14
  • 最后修改日期:2024-04-18
  • 录用日期:2024-04-18
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