[Objective] To investigate the effects of different light qualities on the growth, photosynthesis, flavonol accumulation and antioxidant capacity of Ginkgo biloba, and to screen out the more suitable light qualities for the growth and flavonol accumulation of Ginkgo biloba, so as to provide theoretical basis for the improvement of Ginkgo biloba"s medicinal value. [Methods] Two-year-old Ginkgo biloba seedlings were used as test materials, and four light quality treatments with red and blue ratios of 1 : 1 ( 1R1B ), 1 : 3 ( 1R3B ), 1 : 5 ( 1R5B ) and white light ( W, control ) were set up. After 20 days of treatment, the growth, photosynthetic physiology, flavonol content and antioxidant capacity of Ginkgo biloba were analyzed. [Results] Different ratios of red and blue light reduced Ginkgo biloba biomass and were detrimental to plant height and ground diameter compared to white light. 1R5B treatment could promote the increase of leaf area, total chlorophyll content and Ci. The Tr and Gs of leaves and the total biomass of Ginkgo biloba were increased by 1R1B treatment. The contents of Pn, Fm, Fv / Fm, Fv / Fo, qP, carotenoids, total flavonols and antioxidant capacity of leaves under 1R3B treatment were the highest. [Conclusion] Different proportions of red and blue light had an effect on the growth and photosynthetic characteristics of Ginkgo biloba, and significantly increased the content of flavonols and antioxidant capacity. Comprehensive analysis showed that 1R3B treatment was most conducive to the accumulation of flavonoids in Ginkgo biloba, which was the ideal light quality.