Establishment of a high efficient regeneration system in vitro and transformation mediated by Agrobacterium rhizogenes in Buckwheat (Fagopyrum esculentum)

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Establishment of a high efficient regeneration system in vitro and transformation mediated by Agrobacterium rhizogenes in Buckwheat (Fagopyrum esculentum)
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The hypocotyls of buckwheat (Fagopyrum esculentum Moench.) seedlings were sliced and cultured on MS medium containing different phytohormones to induce callus.The frequency of callus induction reached to 100%.Calli were subcultured on MS medium supplement

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JIN Hong, JIA Jing-fen *,HAO Jian-guo. Establishment of a high efficient regeneration system in vitro and transformation mediated by Agrobacterium rhizogenes in Buckwheat (Fagopyrum esculentum)[J]. Acta Botanica Boreali-Occidentalia Sinica,2002,22(3):-616,T013

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