Cloning Strategy of Barnase Gene (Extracelluar Ribonuclease Gene)
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Abstract:
Two barnase-cloning methods currently reported,independent cloning and barstar gene-protected cloning of barnase gene were comparatively examined.When four plasmids were respectively used to independently clone barnase gen,a small number of positive clone
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SONG Hong-yuan, CAO Bi-hao, DING Jian-gang, LEI Jian-jun, SONG Ming . Cloning Strategy of Barnase Gene (Extracelluar Ribonuclease Gene)[J]. Acta Botanica Boreali-Occidentalia Sinica,2005,25(6):