Cloning of Lipoxygenase cDNA Fragment of Pear (Pyrus pyrifolia ) and Construction of Its Corresponding RNAi Vector
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Abstract:
The total RNA was isolated from ripen fruit of pear(Pyrus pyrifolia Nakai 'Wakahikari').A specific fragment of the expected size was amplified by Reverse Transcription-Polymerase Chain Reaction with degenerate oligonuclectide primers designed on the basis
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HU Zhong-dong, QIAO Yu-shan, WANG San-hong, YAO Quan-hong, ZHANG Zhen. Cloning of Lipoxygenase cDNA Fragment of Pear (Pyrus pyrifolia ) and Construction of Its Corresponding RNAi Vector[J]. Acta Botanica Boreali-Occidentalia Sinica,2007,27(7):