With the methods of artificial cultivation and paraffin technology, It was observed that the morphogenesis of antheridium in Onoclea sensibilis and the multiformity of growing location. It is the first reported that the archegonium of overripe gametophyte shriveled and then disappeared, but the antheridium is normal, and the morphogenesis of the rachis contains protuberance, hollow and layering. The growing locations of antheridiums are different. For example, some are growth in the outer surface of the rachis or immersed in the rachis, some are growth in the hollow inner surface of the rachis. The structures of antheridium are also various. This article disscussed that phylogenetic significance on the morphogenesis of antheridium in Onoclea sensibilis and the multiformity of growing location and supplied basic data for discussing the systemic evolution of related groups.