A full-length cDNA of COMT,which is closely related to the biosynthesis of galanthamine in Amaryllidacea plants,was first successfully cloned from Lycoris radiata by the method of homology cloning and RACE,and was named as LrCOMT.The nucleotide analysis showed that the cDNA sequence of LrCOMT gene had 1 246 bp,containing a 1 101 bp open reading frame,which encodes 366 amino acid residues.Amino sequence analysis demonstrated that the deduced amino sequence of LrCOMT has many typical features of COMTs,and shared more than 60% indentity with COMTs from Iris hollandica,Zea mays,Nicotiana tabacum and other plants.Subsequently,the LrCOMT was ligated into pET-29a vector,and transferred into E.coli strain BL21 for heterologous expression.And the result demonstrated that the recombinant protein was induced by IPTG,and its molecular weight is about 40 kD,which is consistent with other COMTs from other plants.