We choose Columbia wild type Arabidopsis thaliana as the experimental material,using He-Ne laser instrument(632.8 nm,5 mW/mm ²) to handle the seeds,then gather statistics about germination potential,germination percentage,root length,the contents of chlorophyll,soluble sugar,soluble protein and MDA.The results showed that,short period of He-Ne laser radiation could promote the growth of A.thaliana.It has an obvious influence when the time reaches 4 minutes,and the germination potential,germination percentage,root length,the contents of chlorophyll,soluble sugar,soluble protein reach the highest.However,the content of MDA reach the lowest.When the time is beyond 4 minutes,the promotion influence become less,and then it inhibited with longer radiation time.All of these show that suitable He-Ne laser dosage radiation could promote the seeds germination and seedling growth of A.thaliana and protect the cell membrane from being injured.