Ultraviolet spectrophotometry (UV) was used to study the changes of the total flavonoids content in vegetative organs of 2-year-old cultivated Glycyrrhiza glabra L.in different seasons.It was shown that the sequence of total flavonoids content from the highest to the lowest was below:upper leaves,middle leaves,hairy root,horizontal rhizome,lateral root,main root,vertical rhizome,upper stem,middle stem and lower stem.It had a great fluctuation from April to November.The higher content was occurred in June,September and October.These results indicated that total flavonoids content was the highest in the parts of the leaf and the hairy root.It was suitable for 2-year-old cultivated G.glabra L.to harvest in early autumn.Therefore,we suggested that the leaf could be harvested for medicine.