In order to accelerate propagation of superior clones of Catalpa bungei,tissue culture of five clones was studied comparatively,which were 004-1,1-3,2-6,015-1 and 1-4.The results showed that the different clones of Catalpa bungei were the main factors influencing the growth traits of seedling,and the variance components of subculture were during 93.89%~98.16%.Variance analysis showed that the index in different clones were obvious,such as multiplication coefficient,bud number,bud length,leaf number,callus enlargement of the basal stem,and in rooting culture phase.The index such as rooting rate,number of roots,root length,and the variance components were at 0.01 significant level,and variance components were 92.97%,88.75% and 96.25%,respectively.The results indicated that 004-1 was the best of the five clones and the poorest was 1-4.As for 004-1,the multiplication coefficient reached 10.74,the rooting rate was 61.11%,the transplanting survival rate was 74.44%.