Used plasmid pCAMBAR.CHI.11 as donor,the seeds of maize ‘Chang 7-2’,soybean ‘Jinyi 19’ and sorghum ‘Jinliang 5’ as acceptor,bar gene was introduced into crop plants by non-tissue-culture transformation method.The results of PCR amplification and Southern hybridization analysis showed that the transgenic plants derived from wounded-embryo seeds of maize,soybean and sorghum were obtained.The transformation frequency was up to 5%,in contrast,while the transformation frequency of normal seeds was 0.Acetosyringone solution at final concentration of 200 μmol/L in medium could make transformation frequency of maize seeds 8 times higher than that of CK.In addition,the supersonic wave (300 W) treatment further enhanced 60% of transformation frequency.Compare with CK,the ratio of seedling of each crop wound-embryo seeds were reduced greatly,only 17%.The Basta-tolerance activity experiment of 3 crops with wounded-embryo seeds was conducted in the laboratory.The investigation results of the experiment suggested that a little difference of Basta-tolerance activity of 3 crops was existed among them.The critical values of maize,soybean and sorghum that can endured damage of herbicide were 0.15%,0.1% or 0.05%,respectively.The results of Basta-sprayting experiment in the field further also confirmed that the crop rank according to their Basta-tolerance activity was sorghum＜soybean＜maize.However,the crop rank according to their Basta-susceptibility became soybean＜sorghum＜maize.We sprayed Basta solution at different development stage in the field,the results (T₂) showed that,compared with that of spraying Basta at early development stage,the Basta-susceptibility of each crop had a little reduced when spraying Basta at later development stage.Spraying Basta at early stage of growth,the ratio of living seedlings of maize,soybean and sorghum were 57%,49% and 52%,respectively,but spraying Basta at later stage of growth,it became 77%,70% and 73%,respectively.PCR amplification results of all samples come from these material displayed the ratio of PCR positive-result was up to 80%.The result indicated that the difference of the Basta-tolerance activity or Basta-susceptibility of each crop plant resulted in the difference of the ratio of living seedling.The results of PCR amplification and southern hybridization analysis to T₁～T₃ plants showed the target transgene could inheritance steadily.