Seven compounds were isolated from the fruiting bodies of Hydnellum sp.No.2 by silica gel and Sephadex LH-20 column chromatography.With combine analysis of spectral data and literature,their structures were identified as ergosta 7,22-dien-3β-ol (1),ergosterol peroxide (2),(22E,24R)-3β-hydroxy-24-methylcholesta-5,22-dien-7-one (3),nipagin (4),(24R)-ergosta-7,22-diene-3β,5α,6β-triol (5),tuberoside (6),and cerebroside B (7).Compounds 4,5,6 and 7 were obtained from this genus for the first time.