14-3-3 proteins are considered master regulators of many signal transduction cascades in eukaryotes.They can interact with target proteins containing phosphoserine or phosphothreonine to regulate the activities of various enzymes which relate to carbon and nitrogen metabolism,tricarboxylic acid (TCA) cycle,the shikimate pathway and so on.Based on the recent research status on protein discovery,gene identification,structure and function of 14-3-3 proteins in plant are summarized in this paper.Meanwhile,the mechanism of 14-3-3 proteins to interact with the target is described.Furthermore,we bring forward the prospect about the further study on plant 14-3-3 proteins.