Cloning and Expression Analysis of Stilbene Synthase Gene from Chinese Wild Vitis quinquangularis
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    Abstract:

    VqDSTS1,was isolated by reverse transcription-polymerase chain reaction (RT-PCR) from Chinese wild Vitis quinquangularis accession ‘Danfeng-2’,and the sequence and expression model then was analyzed.The results suggested the full length cDNA sequence of VqDSTS1 was 1 179 bp,and the accession number of VqDSTS1 was JQ342086,and encoded 392 amino acid residues.Detailed analysis of the amino acid sequence revealed that VqDSTS1 contained “IPNSAGAIAGN” and “GVLFGFGPGLT” which could be found in all stilbene synthase gene family.With alignment of amino acid sequences,VqDSTS1 shared highly identity between 95.2%~98.7% with the stilbene synthase of other Vitis.From the analysis of semi-quantitative RT-PCR,it was found that the VqDSTS1 expression is introduced by powdery mildew with twin-peak model.This work would make a foundation to investigate the function of stilbene synthase gene family from Chinese wild Vitis quinquangularis accession ‘Danfeng-2’.

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CAO Jiangling, HE Mingyang, ZHU Ziguo, WANG Yuejin. Cloning and Expression Analysis of Stilbene Synthase Gene from Chinese Wild Vitis quinquangularis[J]. Acta Botanica Boreali-Occidentalia Sinica,2012,32(5):859-865

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  • Online: October 11,2012
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