Two pairs of gene specific primers were designed according to the nucleotide sequence of CMe-ERF1 and CMe-ERF2 genes from melon in GenBank.The full-length coding regions of the CMe-ERF1 and CMe-ERF2 cDNA,which were 498 bp and 822 bp respectively,were cloned by RT-PCR from ripening fruit of melon cv.Hetao.Sequence analysis indicated that the cDNA sequences were consistent with that of reported melon CMe-ERFs cDNA.Expression levels of CMe-ERF1 and CMe-ERF2 mRNA were investigated at different development stages of melon fruit by qPCR,and showed that the expression levels of these genes were highly relative to fruit ripening and ethylene production.The results indicate the genes might play important role in fruit ripening.