Cloning and Tissue Expression Analysis of Catalase Gene in Dongzao (Ziziphus jujuba)
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    Abstract:

    The gene fragment homologous with peach CAT1 gene up to 88%,was obtained from the SSH libraries of Dongzao (Ziziphus jujuba cv Dongzao) halfred and red fruit.Based on the fragment,specific primers were designed for 5′ and 3′RACE amplification resulted in a sequence of 1 586,which contains a 1 479 bp complete open reading frame and encodes 492 amino acids,with a conserved functional domain of CAT gene family.It was named ZjCAT,with GenBank accession number JN831452.Amino acid sequence analysis indicated that,ZjCAT was high identity with the catalases of Prunus persica,Gossypium hirsutum and many other plants,and it belonged to the class Ⅲ.The results of realtime PCR showed that the gene differentially expressed in different tissues and at different fruit maturity stages,of which the expression in halfred stage was the highest,indicating its possible important roles in regulating the senescence of Dongzao fruit.

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LIN Xinggu, KONG Decang, PANG Xiaoming, LI Yingyue. Cloning and Tissue Expression Analysis of Catalase Gene in Dongzao (Ziziphus jujuba)[J]. Acta Botanica Boreali-Occidentalia Sinica,2012,32(6):1086-1092

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  • Online: July 06,2012
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