In order to study the effect of different sulfur and nitrogen fertilizer combined application on N transfer and leaf senescence of winter wheat,N content、water and chlorophyll content of 3 winter wheat varieties (‘Yumai 66’ and ‘Weimai 8’ are staygreen wheat,‘Xiaoyan 6’ is nonstay green wheat) were measured.The results showed that under N₁₂₀ treatment,N content of stem and leaf,chlorophyll content of leaf with S₆₀ treatment was lower than that of other two S treatment at anthesis;Leaf water content was lower than that of other two S treatment at the late grain filling stage.Under N₂₂₀ treatment,leaf N content at anthesis,total N accumulation at harvest,N Harvest Index,water content and chlorophyll content of leaves were all higher than that of other two S treatment.The effect of sulphur on wheat depended on the amount of nitrogen application,and S demonstrated a dual character in all 3 winter wheat varieties.On the one hand,under the condition of lowerlevel nitrogen,sulphur had negative effect on the N uptake and senescence of leaf；On the other hand,under the condition of higherlevel nitrogen,sulphur had positive effect on the N uptake,senescence of leaf,grain yield and total biomass.The appropriate amount of N application for high yield was about 220 kg/hm²,and the appropriate amount of S application for high yield was about 60 kg/hm².Highlevel N and S fertilizer delay senescence more in nonstaygreen wheat than that in staygreen wheat.