Primers were designed according to the CBF transcription factors of Peach (Prunus persica),Sweet cherry (Prunus avium) et al from GenBank.Fragments of CBF gene were isolated from Prunus mume by PCR and RTPCR.The CBF gene was 821 bp long,encoding a putative protein of 238 amino acids;The amino acids sequence owns the characteristics of the CBF protein,which contains an AP2/EREB DNAbinding domain and two special short amino acids sequences;Similarity analysis showed that the nucleotide were highly similarity to that of P.avium,P.tenella et al.Relative realtime PCR experiment showed the expression of PmCBF1 was coincidence with the expression characteristics of the CBF gene after exposed to 4℃.The expression of PmCBF1 increased at beginning and achieved the highest after 8 hour,indicating PmCBF1 was induced under low temperature stress.