Isolation and Expression Pattern Analysis of GmABC from Fuyang Soybean
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    Abstract:

    In this study,a member of ABC transporter gene named GmABC,was isolated from Fuyang soybean ( Fuyang Glycine max NO.11) using RACE (rapid amplification of cDNA ends) method.The full length of cDNA was 4 693 bp with a 4 341 bp open reading frame (ORF) which encoded a peptide of 1 447 amino acids and containing the highly conserved ATP binding site modules,and the predicted protein molecular mass was 162.5 kD.The protein sequence alignment showed that this gene encoded a protein belonging to the PDR (pleiotropic drug resistance) family of ABC proteins.The phylogenetic analysis showed that GmABC has the highest similarity with PDR from Medicago truncatula,which shared 84% homology with Medicago truncatula in the amino acid sequence.Expression analysis indicated that GmABC gene was induced by Cd2+,expression level of GmABC gene was the highest at Cd2+ concentration of 100 mg·L-1.The results suggested that GmABC may play an important role in Cd stress resistance of Fuyang soybean.

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ZHAO Hu. Isolation and Expression Pattern Analysis of GmABC from Fuyang Soybean[J]. Acta Botanica Boreali-Occidentalia Sinica,2012,32(9):1726-1730

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  • Online: September 25,2012
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