Five lines of YLP,YLP-1,YLP-7,YLP-9,YLP-13 and YLP-16,the distant hybridization generations from Triticum monococcum and Avena fatua L.,which stability resistance to Puccinia striiformis f.sp.tritici.(Pst) were selected as experiment materials.In order to choose containing alien chromosomes and the strongest resistant plants,we made cytogenetic analysis and resistance evaluation of these hybrid derivatives.2n=42 chromosomes were observed after identification root tip in cytology and the chromosome configuration of most pollen mother cell during metaphase of meiosis Ⅰ were 2n=21Ⅱ;In the pollen mother cell of F₁ plants from five derivatives and Chinese Spring,the frequency of abnormal cells were 16%～50%.Primary identification showed that these materials were translocation lines.YLP were verified generations from distant hybridization.The resistance of these selected nine lines to nine races of stripe rust were done during seedling stage,five lines (YLP-1-4,YLP-7,YLP-9-1,YLP-9-3 and YLP-16-1) resistant to all of these nine races,and could have new genes different from Yr5,Yr10,Yr15 and Yr24/Yr26.We concluded that YLP contains new resistance genes to stripe rust.It could be used as a new source in wheat resistance breeding to stripe rust.