Laser confocal scanning microscope (LCSM) and whole-cell patch clamp were used to reveal the influence of actin cytoskeleton deploymerizing agent cytochalasin B （CB）and stabile reagent phalloidin (PD) on the dynamic of cytosolic free calcium concentration ([Ca²⁺]_i) and calcium ion channels in the protoplasm membrane of Pyrus pyrifolia pollen tube cutting-edge.The results showed that CB could promote [Ca²⁺]_i increase and activate Ca²⁺ channel in plasma membrane;while phalloidin had no effect on [Ca²⁺]_i and did not activate Ca²⁺ channel.The results indicated that disruption of actin cytoskeleton might inhibit pollen germination and tube growth by activating Ca²⁺ channel in plasma membrane and increasing the concentration of [Ca²⁺]_i.