In order to investigate the influence of two bio-organic fertilizers on aroma compounds,5 year old Italian Rieslings grape were treated with 0.02% root application and 0.01% foliage spraying,respectively.Solid phase microextraction (SPME) and gas chromatographymass spectrometry (GC/MS) were used for determination after fruit mature.The results showed that:(1)The detection aroma compounds were 33,28,31 and 27 with 2 bio-organic fertilizers and 2 treatments,respectively.They are all higher than 22 kinds of the CK;but there were no significant effects of the detection rate on aromatic compounds.(2)Using bio-organic fertilizer had no significant effects on 2 kinds of aromatic compounds,which had the most relative content including 2-hexenal,hexanal,2-hexen-1-ol and 1-hexanol,but it could significantly increase the ralative content of nerol,linalool,terpineol and damascenone,which were related with the special aroma compounds.The root application with 0.02% of bio-organic fertilizerⅠwas the best;The relative content of nerol,linalool and damascenone were 320.0%,485.7%,860.0% higher than that of CK respectively;the relative content of terpineol was 0.63%,which was not detected in CK.(3)Using bio-organic fertilizers could significantly increase the relative content of ketone,ester,acid,benzene derivatives and alkenes compounds in grape berry,all treatments were higher than that of CK.