Five different genotypes of kale (Brassica oleracea L.var.acephala) were used to study the relationship of microspore developmental stage with bud size,effects of genotype on embryogenesis,embryos transferred to plantlets,chromosome doubling and ploidy identification.The results showed that:(1)The bud size suitable for using as index in microspore culture was 3.5～4.5 mm length and the ratio of anther to petal was 0.85～1.10.(2)In 5 genotypes,the embryogenesis efficiency of ‘4105’ was the best,that was 2.45 embryos per bud,then ‘7341’ with 2.18 embryos per bud;the efficiency of ‘7340’ and ‘7348’ was lower,that was 1.36 and 0.51 embryos per bud,respectively;‘7342’ had no embryos produced.(3)The microspore-derived seedlings were treated with 150 mg·L^-1 colchicine for 24 h,then used to identify the ploidy level with flow cytometry.We found that the frequency of total doubling diploids was 78.4%,which was better for utilization in production.