In this experiment,Casuarina equisetifolia was inoculated with different mycorrhizal fungi shoots under low temperature stress.The relationship between cold resistant physiological indicators and inoculation of mycorrhizal fungi of C.equisetifolia was analyzed in order to investigate the response characteristics of C.equisetifolia inoculated with mycorrhizal fungi under low temperature stress,and provide the basis for scientific evaluation and improvement of cold tolerance of C.equisetifolia.The results show that:(1)Inoculated with mycorrhizal fungi could significantly promote the growth of C.equisetifolia seedlings.(2)With the reduction of the stress temperature,SOD,POD,CAT activities of inoculated with mycorrhizal fungi of C.equisetifolia twigs were all increased and then decreased trend.MDA content and cell membrane permeability increased with decreasing temperature.(3)Compared with the unvaccinated control at same temperature the activities of SOD,POD,CAT of seven inoculated twigs of C.equisetifolia were significantly increased (maximum when 0～4℃) and a significant reduction in MDA content and cell membrane permeability,moreover there were significant difference between treaments.Study found that inoculated under appropriate low temperature stress with mycorrhizal fungi could significantly improve the protection of enzyme activity,reduce cell membrane permeability and MDA content of C.equisetifolia twigs to improve their resistance to cold.Inoculated AMF Glomus caledonium Nicoison & Ceelemann and ECMF Scleroderma polyrhizum Pers had the best promoting effect on C.equisetifolia cold resistance.