Potted flue-cured tobacco variety ‘K326’ was used as experimental material to explore influence of light quality on carbohydrates and activity of some enzymes in the process of maturation and botanical characters.Results showed that compared with CK,invertase activity in leaves decreased under red films treatment,while amylase activity increased,contents of starch and reduced sugar increased;activity of invertase and amylase in leaves were lower under blue film treatment,contents of starch and reduced sugar were higher;and under green film treatment,invertase activity in leaves increased,amylase activity decreased,contents of starch,total sugar,and reduced sugar decreased.The growth status of performance for the red film is better and the worst is green film.There is no difference between CK and blue film.The study found that the red film to better improve the environment for the growth of the tobacco plant,the obvious adjustment the tobacco transfer enzymes and amylase activity for starch and reducing sugar accumulation is conducive to better growth of the tobacco plant.