To find out difference of promoters to drive glgC expression in transgenic plants,glgC expression driven by GBSSⅠand 35S,respectively,in potato transgenic lines was screened in this study.Phenotype on tuber starch accumulation was assayed in transgenic potatoes.The result showed that glgC driven either by GBSSⅠor 35S did express in the transgenic lines.AGPase activity,tuber starch contents and starch viscosity in the transgenic lines expressing single copy of glgC were significantly higher than those in control.Tuber starch contents and starch viscosity in GBSSⅠ∶glgC line were 2 time higher than those in control.Amylose contents in GBSSⅠ∶glgC line was significantly lower than that in control.The result indicated that starch biosynthesis can be regulated by glgC expression driven by different promoters.