The authenticity of 239 interspecific cross progenies from Vitis riparia 580×Vitis amurensis 0936 was identified by SSR markers.Twelve polymorphism primers were selected randomly between two parents by SSR markers amplified,of which 7 primers with specific bands from male parent were applied to identify the authenticity of 239 progenies.The results showed that 161 of 239 progenies were all identified to be true hybrids owing to their specific bands from male parent and morphological analysis of the field.Simultaneously,there was considerable variation in the hybrids,including some with new bands.The results suggest that SSR markers could be used as effective molecular markers for the authenticity,identification and genetic analysis of the progenies of grape.