Methyl Jasmonate (MeJA) with different concentrations was applied to the suspension cultures of Tripterygium wilfordii Hook.f..The results showed that 50～400 μmol/L MeJA inhibited the accumulation of total alkaloids.cDNA-AFLP (cDNA amplified fragment length polymorphism) analysis found 19 differentially expressed genes between suspension cell culture of T.wilfordii Hook.f.with and without elictor treated.Through Blastn analysis with NCBI protein database,the functions of 7 of them were predicted,which involving in the processes of signal transduction,transcription regulation,energy metabolism and so on.These results will facilitate the improvement alkaloids production using biotechnology methods in the long run.