A field experiment was carried out to study the effects of sulfur fertilization on the soil pH,the growth,yield and quality of flue-cured tobacco (Zhongyan 100).Four sulfur rates (0.1,0.2,0.3,and 0.1 with 3.0 kg/m² stillage) were used in this experiment.The results showed that:(1)The pH of root zone soil was significantly reduced by application of 0.1 to 0.3 kg/m² sulfur,comparing with the control.The minimal reduction of pH was more than 0.3 units.(2)Thirty days after transplanting,compare with the control,the dry weight of root,stem and leaf increased 16%～37%,9%～28% and 17%～25%,respectively.Sulfur promoted the early growth of tobacco seedling root,and finally increased the dry matter accumulation of the plant.The yield and the ratio of superior and middle class of tobacco leaves were significantly increased at the sulfur application of 0.2 kg/m² and 0.1 kg/m² with 3.0 kg/m² stillage,comparing with the control.(3)Sulfur increased total nitrogen of cured leaves,whereas it reduced the educing sugars/nicotine ratio.The sulfur application of 0.1～0.2 kg/m² significantly increased total nitrogen and nicotine of cured leaves,and 0.1 kg/m² sulfur with 3.0 kg/m² stillage could had more remarkable effect.However,the application of 0.3 kg/m² sulfur did not work well.The results suggested that sulfur could significantly reduce the pH of root zone soil,improve the growth,yield and quality of flue-cured tobacco.The application of sulfur at a rate of 0.2 kg/m² and 0.1 kg/m² with 3.0 kg/m² stillage were the effective way to ameliorate soil acidity and increase the yield and quality characteristics of flue-cured tobacco in alkaline soil.