By using grinding method,we successfully separated and purified the endophyte bacteria from the surface disinfected Nepenthes mirabilis organs.Then we respectively used casein plate hydrolysis spot method and shaking flask culture method to conduct primary screening and secondary screening tests to the endophyte bacteria,following by the 16S rDNA identification method to the isolated bacteria strains.As a result,from the leaves,ampulla,roots and stems of N.mirabilis,25 endophytes were isolated,among which the A3 and L5 could produce extracellular protease.A3 had a stronger ability to produce protease:the D/d value of A3 was 6.5,and the protease activity of the fermentation broth was 17.58 U/mL;while,the D/d value of L5 was 3.0 and the protease activity of the fermentation broth was 15.77 U/mL;furthermore,both A3 and L5 showed 99% homologous to Bacillus.Between the two endophytes,A3 was the Bacilus subtilis and L5 might be one new subspecies or new varieties of the B.subtilis.