In order to identify parasitic property and to provide foundation for construction and optimization of manual propagation technology of Cistanche deserticola,we studied the medium,method and surroundings condition of in vitro parasitism with aseptic seeding of Haloxylon ammodendron and germinate seed of C.deserticola.The results showed that glass fiber filter was suitable culture medium of in vitro system parasitism of C.deserticola.Under condition of dark,relative humidity at 80% and temperature between 18℃~30℃,germinated seed of C.deserticola parasite H.ammodendron for 8 days,and the highest parasitism rate was 82%.But germinated seed could not parasite H.ammodendron under light condition.