Using RT-PCR and RACE,we obtained the spinach 14-3-3 protein gene full-length cDNA sequence (GenBank accession No.JX952165),and designated So14-3-3.The full length sequence was 1 166 bp,with the ORF of 801 bp,which encodes 266 amino acids.Amino sequence alignment showed that So14-3-3 had high identity with other plants 14-3-3.The expression of So14-3-3 in root and leaf increased with the increasing nitrate treatment time and NO₃^- concentration by RT-PCR.The prokaryotic expression vector pGEX4T-So14-3-3 was constructed.A recombinant protein induced by IPTG with a molecular weight of 56kD was obtained.Further,Western blotting analysis showed that the expression of So14-3-3 also increased with the increasing nitrate treatment time and NO₃^- concentration.These results provide experimental base to further study the function of So14-3-3.