With the aim of molecular marker assisted selection for seed width in pumpkin (Cucurbita maxima),193 progenies of F₂ generation derived from a cross between the large seed variety ‘0515-1’ and the small seed variety ‘0460-1-1’ in the present study were screened using AFLP and SSR molecular markers for their polymorphic sites,and a genetic linkage map containing 84 polymorphic loci was constructed for identifying seed width trait.The genetic linkage map consisted of 12 linkage groups,and was,totally,683.50 cM in length.The average distance between adjacent loci was 8.13 cM.Further analysis using the composite interval mapping method showed that 4 QTL spread over the 3 linkage groups,the percentage of phenotypic variation explained by individual QTL ranged from 2.87% to 29.68%.