Primers were designed according to the cDNA sequence of tea alcohol dehydrogenase gene (CsiADH1).RT-PCR method was used to clone CsiADH1 from ‘Longjing 43’.The expression levels of CsiADH1 under biotic and abiotic stress were analyzed by qRT-PCR.The results showed CsiADH1 contains an open reading frame of 1 044 bp which encodes a protein of 347 amino acids.Tea geometrid feeding,wounding,JA and SA treatment up-regulated the expression levels of CsiADH1.Using molecular cloning techniques,the ORF region sequence was cloned into the pCAMBIA1301 vector under the control of a consistent promoter CaMV35S.The recombinant pCAMBIA-ADH was then inserted into tomato cultivar‘Zhongshu 4’using Agrobacterium tumefaciens-mediated transformation.8 transgenic plants were obtained by PCR identification.The results will help to further understand the molecular mechanism of CsiADH1 in plant induced defense response.