Resistance to Salt in Cabbage (Brassica oleracea L.var.capitata L.) Transformed with StP5CS Gene
CSTR:
Author:
Affiliation:

Clc Number:

Fund Project:

  • Article
  • |
  • Figures
  • |
  • Metrics
  • |
  • Reference
  • |
  • Related
  • |
  • Cited by
  • |
  • Materials
  • |
  • Comments
    Abstract:

    In order to study the possibility of utilizing StP5CS gene to enhance the resistance to salt,we introduced StP5CS gene into hypocotyls of cabbage (Brassica oleracea L.var.capitata L.) by Agrobacterium-mediated transformation.After selection with bialaphos,36 transgenic plants were regenerated.PCR and Southern hybridization analysis revealed that StP5CS gene had been integrated into the genome of cabbage plants.Moreover,RT-PCR analysis showed that StP5CS gene was expressed in the most transgenic plants.The results of NaCl treatment indicated that the transgenic plants could survive while CK could not.The SOD activity,proline content and rate of electrolyte leakage of transgenic plants increased with the increase of the NaCl concentration.They were highest when the transgenic plants were treated with 400 mmol/L NaCl.The results suggested that the salt tolerance of the transgenic plants increased.

    Reference
    Related
    Cited by
Get Citation

WANG Xiaohong, ZENG Aisong, GAO Bing, SONG Lixiao, YAN Jiyong. Resistance to Salt in Cabbage (Brassica oleracea L. var.capitata L.) Transformed with StP5CS Gene[J]. Acta Botanica Boreali-Occidentalia Sinica,2013,33(5):885-891

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Received:
  • Revised:
  • Adopted:
  • Online: June 05,2013
  • Published:
Article QR Code