A protein kinase gene (calcineurin B-like proteins-interacting protein kinase,CIPK) was cloned from barely drought resistance variety ‘Ganpi 7’ by RT-PCR technology and named as HvCIPK1(GenBank accession JX679077).The length of the HvCIPK1 cDNA sequence is 1359 bp,and the predicted protein has 452 amino acids with an estimated molecular mass of 51.05 kD and an isoelectric point of 9.13.The protein contain an N-terminal kinase catalytic domain with a putative activation loop located between the conserved DFG and APE sequences,and the conserved 24-amino acid motif within the C-terminal non-kinase region of the CIPKs.Analysis of the gene expression by quantitative real-time RT-PCR (qRT-PCR) showed that the transcripts of HvCIPK1 were up-regulated in young barley leaves after stress treatments of polyethylene glycol,high NaCl and abscisic acid.The study suggested that the HvCIPK1 was involved in multiple stress response pathways in plants,revealing its key role in abiotic stress responses.