Ten compounds were isolated from the culture of an endophytic fungus Aspergillus sp.12Y03 in Perilla frutescens.Their structures were elucidated by spectroscopic methods as cyclo-(Pro-Gly)(1),cyclo-(Ser-Pro)(2),cyclo-(Ser-4-OH-Pro)(3),cyclo-(Ala-4-OH-Pro)(4),cyclo-(Gly-Phe)(5),cyclo-(Gly-Ala)(6),linoleic acid(7),α-linolenic acid(8),cerevisterol(9) and 22E,24R-5α,6α-epoxyergosta-8(14),22-diene-3β,7α-diol(10).All compounds were isolated from this fungus for the first time.Compound 3 and 4 was shown to be moderate active toward brine shrimp larvae.